Table 5.
Inter-assay (between plates) variations for calibration curve concentrations.
| Inter-Assay Variation (%CV) | ||||
|---|---|---|---|---|
| Cytokine Concentration [pg/mL] | IL-2 | IL-6 | IL-10 | IL-12p40 |
| 5000 | 32.8 | 5.1 | 0.5 | 37.5 |
| 2500 | 23.2 | 13.9 | 1.5 | 13.7 |
| 1250 | 55.9 | 5.8 | 2.2 | 28.0 |
| 625 | 17.8 | 3.0 | 4.3 | 27.8 |
| 312.5 | 10.8 | 4.9 | 5.8 | 27.7 |
| 156.3 | 9.2 | 8.0 | 5.0 | 27.9 |
| 78.1 | 5.5 | 10.0 | 15.4 | 28.0 |
| 39.1 | 5.4 | 13.4 | 23.7 | 27.9 |
| 19.5 | 7.8 | 19.7 | 202.8 * | 29.1 |
| 9.8 | 16.2 * | 54.8 * | 95.7 * | 45.7 |
All values come from OD450-620 signal that was calculated back and then compared with values from calibration curves from other plates and days. *—values below LLOD levels