Table 1.
The molecular determinants of clinical manifestation.
| Protein | Position 1 | Residue 2 | Mean Fst | Domain | Note | |
|---|---|---|---|---|---|---|
| Enc(enc/hem,%) | Hem(hem/enc,%) | |||||
| M | 9 | K(87/22) | R(78/13) | 0.916 | N-terminus | |
| 145 | L(98/0) | M(98/2) | 0.950 | transmembrane region | ||
| E | 76 3 | T(100/0) | A(100/0) | 1.000 | bc loop, domain II | front sheet 4 |
| 130 | H(88/16) | Y(84/12) | 0.958 | e strand, domain II | front sheet | |
| 176 | M(78/22) | L(78/22) | 0.958 | G0H0 loop, domain I | back sheet | |
| 335 | T(77/22) | S(78/22) | 0.937 | BCx loop, domain III | front sheet | |
| 364 | I(100/1) | M(99/0) | 0.989 | DxE loop, domain III | front sheet | |
| 457 | K(100/0) | R(100/0) | 1.000 | transmembrane region | ||
| NS1 | 148 | R(92/0) | K(100/8) | 0.926 | “wing” domain | |
| 161 | V(99/0) | M(99/0) | 0.976 | “wing” domain | ||
| 262 | S(84/22) | A(78/16) | 0.937 | C-terminal domain | antibody binding region | |
| 274 | I(80/22) | L(78/19) | 0.950 | C-terminal domain | ||
| NS2a | 52 | R(62/0) | T(100/0) | 0.943 | ||
| 155 | L(90/17) | Y(78/0) | 0.926 | |||
| NS2b | 33 | V(89/8) | A(92/0) | 0.947 | ||
| 63 | E(99.4/0) | D(100/0) | 0.99 | |||
| NS3 | 314 | K(89/15) | R(85/11) | 0.958 | helicase domain | motif III |
| 404 | D(77/22) | E(78/22) | 0.947 | helicase domain | motif V | |
| 584 | R(96/8) | K(92/4) | 0.958 | helicase domain | ||
| NS4a | 56 | M(87/22) | V(78/13) | 0.916 | ||
| NS4b | 54 | I(86/22) | M(78/14) | 0.916 | ||
| 208 | L(100/0) | V(80/0) | 0.947 | |||
| NS5 | 20 | K(68/24) | R(76/32) | 0.916 | MT domain | near the GTO binding site |
| 31 | I(90/18) | V(82/10) | 0.926 | MT domain | near the GTO binding site | |
| 44 | R(96/7) | K(93/3) | 0.919 | MT domain | ||
| 113 | K(84/7) | R(93/16) | 0.916 | MT domain | near the active MT site | |
| 162 | K(75/22) | R(78/25) | 0.958 | MT domain | near the active MT site | |
| 226 | T(100/0) | S(100/0) | 1.000 | MT domain | near the RNA binding site 219 | |
| 260 | V(82/22) | T(78/14) | 0.920 | MT domain | ||
| 290 | E(99.6/0) | D(100/0.4) | 1.000 | extension structure | ||
| 404 | K(78/22) | R(78/22) | 0.958 | fingers subdomain | ||
| 590 | I(80/22) | V(78/20) | 0.958 | palm subdomain | ||
| 696 | H(78/22) | P(78/22) | 0.950 | inter-domain interface | binding the STAT2 protein | |
| 854 | K(96/0) | R(100/4) | 0.947 | thumb subdomains | ||
| 872 | K(96/4) | R(96/4) | 0.979 | thumb subdomains | ||
| 890 | D(99/0) | E(100/0) | 0.960 | thumb subdomains | ||
1 The protein positions are given according to the TBEV strain SofinKSY (AEP25267.2); 2 The proportion (%) of a dominant amino acid (aa) residue in a determinant site for encephalitic (Enc) and hemorrhagic (Hem) viruses. In parenthesis, proportions are given via “/” for the target group in comparison with the opposite one to illustrate homoplasy. See the full list of site polymorphism at Table S1 and the consolidated alignment (https://doi.org/10.6084/m9.figshare.21154489, accessed on 1 October 2022); 3 The sites with Fst = 1.0 are bolded; 4 Spatial disposition relative to the virion surface.