Figure 6.

MCC950 was able to inhibit MnCl₂-induced pro-inflammatory cytokines detected by qPCR. N2a cells were pre-treated for 1 h with MCC950 (0.1, 1 and 10 µM) and later exposed to MnCl₂ (500 µM) for 24 h. (a) relative TNFα gene expression, (b) relative IL-18 gene expression, (c) relative IL-1β gene expression. All mRNA values were normalized to the housekeeping mRNA, GAPDH, and expressed as a fold-induction over the control sample (set at a value of 1). Data represented as mean ± SEM. * p < 0.05, ** p < 0.001, *** p < 0.0001 vs. control group.