TABLE 1.
Impact of triazole, xanthine oxidase, xanthine, or combinations thereof on Cape buffalo and bovine serum trypanocidal activity
| Serum (45% [by vol] in PBS-G | Supplementa and/or treatment | Trypanocidal activity |
|---|---|---|
| Cape buffalo | None | No |
| Xanthine | No | |
| Triazole | No | |
| Xanthine + triazole | Yes | |
| Catalase depleted | No | |
| Catalase depleted + xanthine | Yes | |
| Catalase depleted + xanthine + catalase | No | |
| Cow | None | No |
| Xanthine | No | |
| Triazole | No | |
| Xanthine + triazole | No | |
| Xanthine oxidase | No | |
| Xanthine oxidase + triazole + xanthine | Yes | |
| Catalase depleted | No | |
| Catalase depleted + xanthine | No | |
| Catalase depleted + xanthine oxidase + xanthine | Yes |
a
Xanthine, 12.5 μM xanthine; triazole, 100 mM triazole; xanthine oxidase, 50 μg of cow milk xanthine oxidase/ml; catalase depleted, rendered devoid of catalase by immunoaffinity chromatography; catalase, 2 mg of catalase/ml.