Table 3.

Advantages and Disadvantages of Enzymatic and Mechanical Stromal Vascular Fraction Separation Methods.

	Enzyme Decomposition	Mechanical Separation
Initial amount of adipose tissue	300 mL ↑	60 mL ↓
Incubation	(+)	(−)
Washing	(+)	(−)
Centrifuge	(+)	(−)
Device	(+)	(−)
Disposable consumable	(+)	(+)
Reliability	(+/−)	(+)
Bacterial contamination	(+/−)	(−)
Enzyme-related side effects in tissue	(+)	(−)
Implementation cost	↑	↓
Duration of implementation	2 h ↑	1 h ↓
Number of cells	↑	↓
Cell surface marker	↓	↑