Table 2.
Top 10 most down and upregulated differentially expressed genes (DEGs) in non-nodulated Casuarina glauca plants supplied with KNO3 (KNO3+), grown at 400 mM NaCl relative to the control (0 mM NaCl), ordered by log2 fold-change (log2FC). NBCI gene symbol, protein name, log2FC and respective Gene Ontology (GO) molecular function(s) and biological process(es) were retrieved from UniprotKB database. Protein names coloured in blue are also among the top 10 most down or upregulated DEGs, respectively, at 400 mM NaCl in plants nodulated by nitrogen-fixing Frankia casuarinae Thr (NOD+).
| Gene | Protein Name | Log2FC | Molecular Function/Biological Process |
|---|---|---|---|
| Downregulated | |||
| XCP1 | Cysteine protease XCP1 | −6.883 | Programmed cell death; proteolysis |
| AT3G10080 | Germin-like protein subfamily 3 member 2 | −6.658 | Manganese ion binding; nutrient reservoir activity |
| ACA13 | Putative calcium-transporting ATPase 13, plasma membrane-type | −6.476 | ATP, calmodulin and metal ion binding; ATPase-coupled cation and calcium transmembrane transporter activity |
| RL5 | Protein RADIALIS-like 5 | −5.931 | DNA binding |
| ERF020 | Ethylene-responsive transcription factor ERF020 | −5.644 | Ethylene-activated signaling pathway; response to chitin |
| YAH3 | Histone H3.2 | −5.476 | DNA binding; protein heterodimerization activity |
| LRX2 | Leucine-rich repeat extensin-like protein 2 | −5.157 | Cell wall organization |
| PATL4 | Patellin-4 | −5.129 | Cell cycle and division; cellular response to auxin stimulus; auxin polar transport |
| PGL3 | Polygalacturonase 1 beta-like protein 3 | −5.073 | Cell size determination |
| FLA12 | Fasciclin-like arabinogalactan protein 12 | −5.055 | Plant-type secondary cell wall biogenesis |
| Upregulated | |||
| WAXY | Granule-bound starch synthase 1, chloroplastic/amyloplastic | 3.459 | Starch biosynthetic process |
| HSP70 | Heat shock 70 kDa protein | 3.363 | Cellular response to unfolded protein; chaperone cofactor-dependent protein refolding |
| N/A | Myrcene synthase, chloroplastic | 3.129 | Magnesium ion binding; myrcene synthase activity |
| SDI1 | Protein sulfur deficiency-induced 1 | 3.000 | Cellular response to sulfur starvation; regulation of glucosinolate biosynthetic process and sulfur utilization |
| UGT87A1 | UDP-glycosyltransferase 87A1 | 2.824 | UDP-glycosyltransferase activity |
| ARF16 | Auxin response factor 16 | 2.644 | Auxin-activated signaling pathway; cell division; pattern specification process; response to auxin; root cap development |
| LECRK111 | Putative L-type lectin-domain containing receptor kinase I.11 | 2.369 | Defense response to bacterium and oomycetes |
| TOGT1 | Scopoletin glucosyltransferase | 2.290 | Identical protein binding; scopoletin glucosyltransferase activity; UDP-glycosyltransferase activity |