Table 5.
Top 10 most down and upregulated differentially expressed genes (DEGs) in Casuarina glauca nodulated by nitrogen-fixing Frankia casuarinae Thr (NOD+), grown at 600 mM NaCl relative to the control (0 mM NaCl), ordered by log2 fold-change (log2FC). NBCI gene symbol, protein name, log2FC and respective Gene Ontology (GO) molecular function(s) and biological process(es) retrieved from UniprotKB database. Protein names coloured in blue are also among the top 10 most down or upregulated DEGs, respectively, at 600 mM NaCl in non-nodulated plants supplied with KNO3 (KNO3+).
| Gene | Protein Name | Log2FC | Biological Process |
|---|---|---|---|
| Downregulated | |||
| RL5 | Protein RADIALIS-like 5 | −7.794 | DNA binding |
| CCD8 | Carotenoid cleavage dioxygenase 8, chloroplastic | −6.134 | auxin polar transport; carotene catabolic process; leaf morphogenesis; response to auxin; secondary shoot formation; strigolactone biosynthetic process Xanthophyll catabolic process |
| TAO1 | Disease resistance protein TAO1 | −5.727 | defense response to bacterium; defense response to bacterium, incompatible Interaction; signal transduction |
| N/A | Lectin | −5.643 | Mannose binding |
| pod | Peroxidase 15 | −5.529 | Hydrogen peroxide catabolic process; response to oxidative stress |
| GLR2.8 | Glutamate receptor 2.8 | −5.247 | calcium ion transport; calcium-mediated signaling; cellular response to amino Acid stimulus |
| LAC22 | Laccase-22 | −5.183 | Iron ion homeostasis; iron ion transport; lignin catabolic process |
| COBL4 | COBRA-like protein 4 | −5.087 | cellulose microfibril organization; plant-type cell wall biogenesis; plant-type cell wall cellulose biosynthetic process; plant-type cell wall organization; plant-type Secondary cell wall biogenesis |
| LECRK91 | L-type lectin-domain containing receptor kinase IX.1 | −5.087 | defense response; defense response to bacterium; defense response to oomycetes; positive regulation of cell death; positive regulation of hydrogen peroxide Metabolic process |
| LRK10L-1.4 | Leaf rust 10 disease-resistance locus receptor-like protein kinase-like 1.4 | −5.058 | Cellular response to hypoxia; phosphorylation |
| Upregulated | |||
| At3g18200 | WAT1-related protein At3g18200 | 2.906 | Transmembrane transporter activity |
| TIC214 | Protein TIC 214 | 2.906 | Protein transport |
| SAT | Stemmadenine O-acetyltransferase | 2.700 | Alkaloid metabolic process |
| DTX56 | Protein DETOXIFICATION 56 | 2.929 | Cellular response to carbon dioxide; regulation of stomatal opening |
| MAKR6 | Probable membrane-associated kinase regulator 6 | 2.861 | Abscisic acid-activated signaling pathway |
| HSP70 | Heat shock 70 kDa protein | 2.728 | Cellular response to unfolded protein; chaperone cofactor-dependent protein refolding; protein refolding; response to unfolded protein |
| HSP22.7 | 22.7 kDa class IV heat shock protein | 2.357 | Endoplasmic reticulum lumen |
| SDI1 | Protein sulfur deficiency-induced 1 | 4.087 | Cellular response to sulfur starvation; regulation of glucosinolate biosynthetic process; regulation of sulfur utilization |
| PEPR1 | Leucine-rich repeat receptor-like protein kinase PEPR1 | 4.584 | Immune response; innate immune response; response to jasmonic acid; response to wounding |
| At1g48100 | Polygalacturonase At1g48100 | 2.495 | Carbohydrate metabolic process; plant-type cell wall modification involved in multidimensional cell growth |