Table 6.
Significantly enriched Gene Ontology (GO) terms, identified by Gene Set Enrichment Analysis (GSEA) of downregulated differentially expressed genes (DEGs) in non-nodulated Casuarina glauca plants supplied with KNO3 (KNO3+) or nodulated by nitrogen-fixing Frankia casuarinae Thr (NOD+), grown at 400 mM NaCl, relative to control (0 mM NaCl). GO aspect, GO ID, GO Name, False Discovery Rate (FDR), intersection size, effective domain size and main gene intersections were retrieved from g:GOSt functional profiling of the gProfiler website, within a threshold of FDR < 0.05.
| Aspect | GO ID | GO Name | FDR | Intersection Size | Effective Domain Size | Intersections |
|---|---|---|---|---|---|---|
| KNO3+ | ||||||
| MF | GO:0008194 | UDP-glycosyltransferase activity | 0.02845 | 6 | 21,106 | GATL2 |
| BP | GO:0071555 | cell wall organization | 0.00081 | 9 | 23,362 | LRX2 |
| GO:0045229 | external encapsulating structure organization | 0.00134 | 9 | 23,362 | LRX2 | |
| GO:0050829 | defense response to Gram-negative bacteria | 0.00667 | 3 | 23,362 | EDS1 | |
| GO:0060866 | leaf abscission | 0.02170 | 2 | 23,362 | EDS1 | |
| GO:0000271 | polysaccharide biosynthetic process | 0.04131 | 5 | 23,362 | GATL2 | |
| CC | GO:0071944 | cell periphery | 0.00013 | 24 | 21,026 | XCP1 |
| GO:0046658 | anchored component of plasma membrane | 0.01625 | 4 | 21,026 | FLA10 | |
| NOD+ | ||||||
| MF | GO:0004352 | glutamate dehydrogenase (NAD+) activity | 0.01952 | 1 | 21,106 | GDH2 |
| BP | GO:0016115 | terpenoid catabolic process | 0.02074 | 2 | 23,362 | CYP707A2 |
| CC | GO:0005886 | plasma membrane | 0.00174 | 10 | 21,026 | GDH2 |
| GO:0071944 | cell periphery | 0.00842 | 10 | 21,026 | GDH2 | |