Figure 3.

Results of the MTT cell viability test accomplished with SH-SY5Y cells with aqueous and ethyl acetate fractions of the total ethanolic extract of G. rivale L. Cells were treated for 24 h (A) and 48 h (B) in the absence (control) and presence of the G. rivale L. fractions of total extract with (grey) and without (white) supplementation of 25 µmol/L Aβ25–35. The assay was performed in quadruplicates. Mann–Whitney test and Bonferroni correction for multiple comparison were applied to address statistically significant differences observed in treated cells: *—p ≤ 0.05 vs. untreated with Aβ25–35 negative control. The controls were supplemented with fresh medium without addition of the fractions of total extract.