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. 2022 Oct 26;11(21):2859. doi: 10.3390/plants11212859

Table 5.

Antioxidant activities assessed by DPPH, TEAC and NBT assays for major individual compounds isolated from the aerial parts of G. rivale L.

Compound DPPH Normalized Activity, % TEAC,
μmol/L Trolox eq./μg
NBT Assay,
nmol of O2•−/min
Kaempferol-3-O-β-D-glucuronide 76.452 ± 0.890 0.519 ± 0.057 7.667 ± 0.96
Isorhamnetin-bis-3,7-O-β-D-glucuronide 75.557 ± 1.784 0.611 ± 0.039 3.477 ± 0.576
3-O-Methylellagic acid 93.675 ± 0.890 2.963 ± 0.024 6.027 ± 0.870
6″-(4-hydroxycinnamoyl)-astragalin 80.827 ± 1.250 0.796 ± 0.024 1.813 ± 0.540
Kaempferol-bis-3,7-O-β-D-glucuronide 99.483 ± 2.512 0.815 ± 0.029 4.649 ± 0.598
Isorhamnetin-3-O-β-D-glucuronide 86.993 ± 1.867 0.019 ± 0.014 7.387 ± 0.563
Caffeoyl malate 80.469 ± 4.012 3.074 ± 0.115 7.076 ± 0.890
DMSO (negative control) - - 8.373 ± 0.076

DPPH—2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay; TEAC—Trolox equivalent antioxidant capacity; NBT (nitroblue tetrazolium) assays—assessment of capacity to scavenge superoxide anion radicals.