Table 1.
Differentially expressed total protein extract from TRF-treated diff-neural cells compared to untreated control.
| Upregulated Proteins | |||||
|---|---|---|---|---|---|
| Uniprot Accession | Description | Symbol | Average Mass | p Value | * Log2 (Fold Change) |
| Q86VP6 | Cullin-associated NEDD8-dissociated protein 1 | CAND1 | 136,375 | 0.021 | 1.287 |
| Q9UL46 | Proteasome activator complex subunit 2 | PSME2 | 27,402 | 0.005 | 0.967 |
| P35637 | RNA-binding protein FUS | FUS | 53,426 | 0.003 | 0.885 |
| O43143 | Pre-mRNA-splicing factor ATP-dependent RNA helicase | DHX15 | 90,933 | 0.010 | 0.666 |
| P49321 | Nuclear autoantigenic sperm protein | NASP | 85,238 | 0.026 | 0.618 |
| P05386 | 60S acidic ribosomal protein P1 | RPLP1 | 11,514 | 0.006 | 0.484 |
| P14174 | Macrophage migration inhibitory factor | MIF | 12,476 | 0.018 | 0.432 |
| O15240 | Neurosecretory protein VGF | VGF | 67,258 | 0.047 | 0.392 |
| P06748 | Nucleophosmin | NPM1 | 32,575 | 0.003 | 0.387 |
| P12236 | ADP/ATP translocase 3 | SLC25A6 | 32,866 | 0.020 | 0.369 |
| P50395 | Rab GDP dissociation inhibitor beta | GDI2 | 50,663 | 0.002 | 0.342 |
| P16401 | Histone H1.5 | HIST1H1B | 22,580 | 0.030 | 0.331 |
| P0DP23 | Calmodulin-1 | CALM1 | 16,838 | 0.025 | 0.326 |
| P0DP24 | Calmodulin-2 | CALM2 | 16,838 | 0.025 | 0.326 |
| P0DP25 | Calmodulin-3 | CALM3 | 16,838 | 0.025 | 0.326 |
| Q00610 | Clathrin heavy chain 1 | CLTC | 191,613 | 0.012 | 0.319 |
| P52209 | 6-phosphogluconate dehydrogenase decarboxylating | PGD | 53,140 | 0.032 | 0.312 |
| P25705 | ATP synthase subunit alpha mitochondrial | ATP5F1A | 59,751 | 0.005 | 0.308 |
| P62841 | 40S ribosomal protein S15 | RPS15 | 17,040 | 0.005 | 0.308 |
| P16104 | Histone H2AX | H2AFX | 15,145 | 0.031 | 0.303 |
| Q71UI9 | Histone H2A.V | H2AFV | 13,163 | 0.007 | 0.296 |
| P0C0S5 | Histone H2A.Z | H2AFZ | 13,553 | 0.007 | 0.296 |
| Q9BUF5 | Tubulin beta-6 chain | TUBB6 | 49,857 | 0.022 | 0.277 |
| Q13509 | Tubulin beta-3 chain | TUBB3 | 50,433 | 0.024 | 0.276 |
| P16403 | Histone H1.2 | HIST1H1C | 21,365 | 0.044 | 0.264 |
| P31150 | Rab GDP dissociation inhibitor alpha | GDI1 | 50,583 | 0.013 | 0.250 |
| P68032 | Actin alpha cardiac muscle 1 | ACTC1 | 42,019 | 0.022 | 0.248 |
| P08238 | Heat shock protein HSP 90-beta | HSP90AB1 | 83,264 | 0.025 | 0.244 |
| P63261 | Actin cytoplasmic 2 | ACTG1 | 41,793 | 0.024 | 0.240 |
| P00558 | Phosphoglycerate kinase 1 | PGK1 | 44,615 | 0.016 | 0.239 |
| Q9BVA1 | Tubulin beta-2B chain | TUBB2B | 49,953 | 0.018 | 0.236 |
| P60709 | Actin cytoplasmic 1 | ACTB | 41,737 | 0.025 | 0.234 |
| P46782 | 40S ribosomal protein S5 | RPS5 | 22,876 | 0.022 | 0.232 |
| P07602 | Prosaposin | PSAP | 58,113 | 0.014 | 0.224 |
| P29401 | Transketolase | TKT | 67,878 | 0.043 | 0.211 |
| P10599 | Thioredoxin | TXN | 11,737 | 0.012 | 0.201 |
| O00571 | ATP-dependent RNA helicase DDX3X | DDX3X | 73,244 | 0.007 | 0.199 |
| P37802 | Transgelin-2 | TAGLN2 | 22,391 | 0.031 | 0.197 |
| P27348 | 14-3-3 protein theta | YWHAQ | 27,764 | 0.023 | 0.185 |
| P62937 | Peptidyl-prolyl cis-trans isomerase A | PPIA | 18,012 | 0.024 | 0.17 |
| P68371 | Tubulin beta-4B chain | TUBB4B | 49,831 | 0.035 | 0.157 |
| P07437 | Tubulin beta chain | TUBB | 49,671 | 0.013 | 0.151 |
| P09936 | Ubiquitin carboxyl-terminal hydrolase isozyme L1 | UCHL1 | 24,824 | 0.043 | 0.145 |
| P33778 | Histone H2B type 1-B | HIST1H2BB | 13,950 | 0.015 | 0.130 |
| P06899 | Histone H2B type 1-J | HIST1H2BJ | 13,904 | 0.015 | 0.130 |
| P23527 | Histone H2B type 1-O | HIST1H2BO | 13,906 | 0.015 | 0.130 |
| P68363 | Tubulin alpha-1B chain | TUBA1B | 50,152 | 0.048 | 0.124 |
| P63104 | 14-3-3 protein zeta/delta | YWHAZ | 27,745 | 0.013 | 0.039 |
| P62136 | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | PPP1CA | 37,512 | 0.040 | 0.037 |
| P62140 | Serine/threonine-protein phosphatase PP1-beta catalytic subunit | PPP1CB | 37,187 | 0.040 | 0.037 |
| P36873 | Serine/threonine-protein phosphatase PP1-gamma catalytic subunit | PPP1CC | 36,984 | 0.040 | 0.037 |
| P07737 | Profilin-1 | PFN1 | 15,054 | 0.004 | 0.037 |
| P10809 | 60 kDa heat shock protein mitochondrial | HSPD1 | 61,055 | 0.034 | 0.025 |
| Down-Regulated Proteins | |||||
| Uniprot Accession | Description | Symbol | Average Mass | p value | * Log2 (Fold Change) |
| P24534 | Elongation factor 1-beta | EEF1B2 | 24,764 | 0.011 | −0.055 |
| P60660 | Myosin light polypeptide 6 | MYL6 | 16,930 | 0.012 | −0.088 |
| Q16555 | Dihydropyrimidinase-related protein 2 | DPYSL2 | 73,503 | 0.012 | −0.121 |
| Q05639 | Elongation factor 1-alpha 2 | EEF1A2 | 50,470 | 0.035 | −0.125 |
| P14625 | Endoplasmin | HSP90B1 | 92,469 | 0.023 | −0.132 |
| P00338 | L-lactate dehydrogenase A chain | LDHA | 36,689 | 0.041 | −0.178 |
| Q00839 | Heterogeneous nuclear ribonucleoprotein U | HNRNPU | 90,585 | 0.017 | −0.195 |
| P30041 | Peroxiredoxin-6 | PRDX6 | 25,035 | 0.035 | −0.200 |
| P39687 | Acidic leucine-rich nuclear phosphoprotein 32 family member A | ANP32A | 19,997 | 0.013 | −0.248 |
| P32119 | Peroxiredoxin-2 | PRDX2 | 21,892 | 0.001 | −0.312 |
| Q08211 | ATP-dependent RNA helicase A | DHX9 | 140,958 | 0.041 | −0.324 |
| P62249 | 40S ribosomal protein S16 | RPS16 | 16,445 | 0.014 | −0.336 |
| P19338 | Nucleolin | NCL | 76,615 | 0.000 | −0.364 |
| Q02878 | 60S ribosomal protein L6 | RPL6 | 32,728 | 0.000 | −0.366 |
| Q06830 | Peroxiredoxin-1 | PRDX1 | 10,676 | 0.033 | −0.394 |
| P11021 | Endoplasmic reticulum chaperone BiP | HSPA5 | 72,333 | 0.005 | −0.405 |
| P09429 | High mobility group protein B1 | HMGB1 | 24,894 | 0.002 | −0.414 |
| P07237 | Protein disulfide-isomerase | P4HB | 57,116 | 0.033 | −0.415 |
| P22626 | Heterogeneous nuclear ribonucleoproteins A2/B1 | HNRNPA2B1 | 37,430 | 0.027 | −0.451 |
| P60842 | Eukaryotic initiation factor 4A-I | EIF4A1 | 46,154 | 0.015 | −0.459 |
| P35232 | Prohibitin | PHB | 22,271 | 0.002 | −0.462 |
| P29373 | Cellular retinoic acid-binding protein 2 | CRABP2 | 15,693 | 0.001 | −0.508 |
| P40939 | Trifunctional enzyme subunit alpha mitochondrial | HADHA | 86,372 | 0.032 | −0.510 |
| P05387 | 60S acidic ribosomal protein P2 | RPLP2 | 11,665 | 0.031 | −0.521 |
| P23396 | 40S ribosomal protein S3 | RPS3 | 26,688 | 0.045 | −0.580 |
| P46781 | 40S ribosomal protein S9 | RPS9 | 22,591 | 0.036 | −0.586 |
| P35579 | Myosin-9 | MYH9 | 226,530 | 0.045 | −0.727 |
| P27824 | Calnexin | CANX | 67,568 | 0.001 | −1.187 |
* Fold change was estimated by dividing the protein expression in TRF treatment with untreated diff-neural cells.