FIG. 3.
WEB 2170 and l-NMMA inhibit PAF-induced NO production. C3H/HeJ-derived peritoneal macrophages were cultured with T. cruzi trypomastigotes (Tc) at a parasite-to-cell ratio of 1:1 with or without PAF (10−7 M) plus WEB 2170 (WEB) (10−5 M), PAF plus l-NMMA (LN) (200 mM), or lyso-PAF (Lyso) (10−7 M). After 48 h, the supernatants were harvested and the nitrite concentration was assayed. Bars represent the mean and SD of triplicate samples. The results are representative of three independent experiments. ∗, P < 0.05 compared with the values obtained with cells cultured in the presence of medium alone.