FIG. 5.

PAF-induced microbicidal activity is decreased by inhibition of the PAF receptor or iNOS activation. C3H/HeJ-derived peritoneal adherent cells were infected with T. cruzi trypomastigotes at a parasite-to-host-cell ratio of 1:1 in the absence of stimulus (Med) or with lyso-PAF (L-PAF) (10⁻⁷ M) or PAF (10⁻⁷ M) with or without WEB 2170 (10⁻⁵ M) (WEB) or l-NMMA (200 mM) (LN) and cultured for 48 h in a humidified chamber containing 5% CO₂ at 37°C. Bars represent the mean and SD of triplicate counts of the number of intracellular parasites found in 100 macrophages. The data are representative of three independent experiments. ∗, P < 0.05 compared to the values obtained with infected cells cultured in medium alone.