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. 2022 Oct 29;23(21):13179. doi: 10.3390/ijms232113179

Figure 5.

Figure 5

The effect of apigenin on the TNAP activity of hFOB 1.19 (squares) and Saos-2 (circles) cells under resting conditions (open squares/circles) or in the presence of stimulators, AA, and β-GP (filled squares/circles). Cells were incubated with different concentrations (µM) of apigenin, and the TNAP activity assay (ALP Yellow pNPP Liquid Substrate System for ELISA) was performed in whole cell lysates after 7 days of culture. Absorbance at λ 405 nm was measured for 60 min with 15 s intervals at 37 °C. The TNAP activity values were normalized to the relative number of viable cells. Data are means ± S.E. of at least three independent experiments (* p < 0.05, **/## p < 0.01 compared with resting/stimulated cells without apigenin).