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. 2022 Oct 29;23(21):13179. doi: 10.3390/ijms232113179

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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TEM images of the co-localization of AnxA6 and TNAP in MVs during the mineralization of hFOB 1.19 (A) and Saos-2 (B) cells under resting conditions or in the presence of stimulators, AA and β-GP. Cells were incubated with different concentrations (µM) of apigenin for 7 days, lysed, and analyzed by TEM (magnification 100,000×). Bar: 200 nm. Additional magnifications 300,000×. AnxA6 was labelled with anti-AnxA6 primary antibody conjugated with 10 nm colloidal gold secondary antibody. TNAP was labelled with anti-TNAP primary antibody conjugated with 5 nm colloidal gold secondary antibody. Sites of AnxA6 and TNAP co-localization are marked by squares for hFOB 1.19 cells and circles for Saos-2 cells.