Figure 6.
Inactivation courses of different octyl-CALB biocatalysts modified with (a) 1% glutaraldehyde, (b) amination using 2 M ethylenediamine, (c) 1 mM picrylsulfonic acid, (d) 10% polyethylenimine, and (e) 10% dextran sulfate. The biocatalysts were inactivated at 68 °C (b) and 75 °C (a,c–e), in presence of 10 mM Tris-HCl buffer at pH 7.0. Other specifications are described in Methods. Unmodified octyl-TLL (empty squares and dashed and red line); octyl-TLL modified with 1% glutaraldehyde (solid rhombus and solid blue line); amination using 2 M ethylenediamine (solid triangles and solid orange line); 1 mM picrylsulfonic acid (solid circles and solid grey line); 10% polyethylenimine (solid squares and solid black line); 10% dextran sulfate (empty rhombus and solid green line).