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. 2022 Oct 22;23(21):12729. doi: 10.3390/ijms232112729

Figure 2.

Figure 2

Adiponectin suppresses NPC cells invasion and migration. (A) Wound healing assay of S18 and 5-8F cells pretreated with adiponectin (0, 10, 20, or 40 μg/mL) for 24 h. Scale bar: 200 μm. Graphs show the relative wound closure. (B) Invasion of S18 and 5-8F cells were evaluated by Transwell invasion assay covered with Matrilgel after treatment with various dosing of adiponectin for 24 h. Scale bar: 200 μm. Graphs show the relative number of invasion cells. (C) Migration of S18 and 5-8F cells were evaluated by Transwell migration assay after treatment with various doses of adiponectin for 24 h. Scale bar: 200 μm. Graphs show the relative number of migration cells. (D) Western blot analysis of E-cadherin, N-cadherin, Slug, Snail, Vimentin, Claudin-1, MMP-2, and MMP-9 in S18 and 5-8F cells after treatment with 40 μg/mL adiponectin for 48 h. Results are presented as mean ± SD of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001, # p < 0.0001.