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. 2022 Oct 22;23(21):12729. doi: 10.3390/ijms232112729

Figure 3.

Figure 3

Adiponectin blocks leptin, IL-6, or TNF-α-induced NPC cell invasion and migration. Transwell migration (A) and invasion (B) assays in S18 and 5-8F cells pretreated with 40 μg/mL adiponectin for 2 h before treatment with 50 ng/mL leptin. Scale bar: 200 μm. Graphs show the relative number of cells. Transwell migration assay in S18 and 5-8F cells pretreated with 40 μg/mL adiponectin for 2 h before treatment with 50 ng/mL TNF-α (C) or 50 ng/mL IL-6 (D). Scale bar: 200 μm. Graphs show the relative number of migration cells. (E–G) Western blot analysis of E-cadherin, N-cadherin, MMP-2, and Vimentin in 5-8F cells pretreated with 40 μg/mL adiponectin in the presence or absence of leptin (50 ng/mL), TNF-α (50 ng/mL), or IL-6 (50 ng/mL) for 48 h. Results are presented as mean ± SD of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001, # p < 0.0001.