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. 2022 Oct 22;23(21):12729. doi: 10.3390/ijms232112729

Figure 5.

Figure 5

AdipoR1 mediates the metastasis inhibitory effect of adiponectin in NPC cells. (A) The mRNA expression of AdipoR1 and AdipoR2 was measured with qRT-PCR in S18 and 5-8F cells after transfected with AdipoR1 or AdipoR2 siRNA. (B) Transwell migration assay in S18 and 5-8F cells treated with 40 μg/mL adiponectin for 24 h after transfected with AdipoR1 or AdipoR2 siRNA. Scale bar: 200 μm. Graphs show the relative number of migration cells. (C) Western blot analysis of E-cadherin, N-cadherin, MMP-9, and MMP-2 in S18 and 5-8F cells treated with 40 μg/mL adiponectin for 48 h after transfected with AdipoR1 siRNA. Results are presented as mean ± SD of three independent experiments performed in triplicate. *** p < 0.001, # p < 0.0001.