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. 2022 Oct 22;23(21):12729. doi: 10.3390/ijms232112729

Figure 6.

Figure 6

AdipoRon inhibits NPC cell migration and invasion in vitro. (A) Wound healing assay of S18 and 5-8F cells treated with AdipoRon (0, 25, or 50 μM) for 24 h. Scale bar: 200 μm. Graphs show the relative wound closure. Transwell (B) invasion and (C) migration of S18, 5-8F cells after treatment with AdipoRon (0, 25, or 50 μM) for 24 h. Scale bar: 200 μm. Graphs show the relative number of cells. (D) Western blot analysis of p-STAT3, cyto-STAT3, nuc-STAT3, cyto-p65, and nuc-p65 in S18 and 5-8F cells after treatment with 50 μM AdipoRon for the indicated time period. (E) Western blot analysis of E-cadherin, N-cadherin, Slug, Snail, Vimentin, MMP-2, and MMP-9 in S18 and 5-8F cells after the treatment with 50 μM AdipoRon for 24 h. Results are presented as mean ± SD of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001, # p < 0.0001.