Table 8.
Summary of tests and accuracy of common commercial yeast identification systems.
| Commercial System | Description of Tests and Controls Included in the Kits | Incubation Condition | Accuracy (%) | Reference |
|---|---|---|---|---|
| API 20 C | 19 carbon assimilation test and 1 control test in 20 strips | 30 °C for 72 h | 98.9 | [88] |
| API Candida | 5 carbohydrate and 7 enzymes colorimetric test in 10 strips | 35 °C for 18–24 h |
97.4 | [89] |
| API 32 C | 29 assimilation tests (carbohydrate, organic acids, and amino acids); 1 negative control, 1 susceptibility test (cycloheximide) and 1 colorimetric test (esculin) in 32 wells. Includes 63 different species in database |
30 °C for 48 h | 92 | [90] |
| Auxacolor system | 13 carbohydrate tests with bromocresol purple, test for cycloheximide resistance and phenoloxidase production in 16 wells. | 37 °C for 48 h | 79.4 | [91] |
| RapID Yeast Plus system | 5 carbon assimilation tests and 13 enzymatic hydrolysis substrate tests | 30 °C for 4 h | 96 | [92] |
| The Uni-Yeast-Tek (UYT) system | 7 carbon assimilation tests, urease, Nitrate and corn meal with Tween 80 agar | 22–26 °C for 2–10 days |
99.8 | [93] |
| MicroScan yeast identification panel | 13 aminopeptidase, 3 carbohydrates, 9 glycosidase, phosphatase and urease tests. | 37 °C for 4 h | 86.9 | [94] |
| VITEK 2 YST | 4 aminopeptidase, 25 carbohydrate, esculin, 3 glycosidase, nitrate, 2 nitrogen, 9 organic acid, and urea tests | 35 °C for 18 h | 94.8 | [95] |