Figure 5.

Overlay of the amine oxidase domains of LOX and LOXL2. (A) Overlay of AlphaFold 2–predicted human LOX (peptide backbone: in white; the LTQ precursors in yellow, His292-X-His294-X-His296: in yellow) and mouse LOXL2 (peptide backbone: in light blue; the LTQ precursors: in cyan, His628, His630, His632: in cyan). The major difference is the secondary structure of the heptapeptide (Val315–Ala321 in human LOX: β–sheet in yellow and Val650–Ala656 in mouse LOXL2: loop in cyan). In both structures, the precursor Lys residues (Lys320 in human LOX and Lys655 in mouse LOXL2) are within 4.0Å and 4.7Å from the C2 position of the precursor Tyr residues (Tyr355 in human LOX and Tyr691 in mouse LOXL2), respectively. (B) Overlay of the precursor Lys–containing heptapeptides, the precursor Tyr–containing peptides and His–X–His–X–His of the Zn²⁺–bound precursor LOXL2 (PDB: 5ze3, in light pink, residues are in magenta, Zn²⁺ is in slate), AlphaFold 2–prediced human LOX (in yellow) and AlphaFold2–predicted mouse LOXL2 (in cyan).