Figure 10.

There is a significant reduction in the number of nodes present in the HUVECs cultured with preeclamptic conditioned media, but this is reversed in Rosiglitazone-treated placentas. HUVECs were cultured with conditioned media on matrigel, and the number of nodes present was calculated by the Image J Angiogenesis Analyzer tool (30). HUVECs cultured with conditioned media from preeclamptic (PE) placentas show significantly reduced number of nodes as compared to healthy control placentas (A, n =6). Conditioned media from Rosiglitazone (Rosi)-treated PE placentas led to a significant increase in the number of nodes present in the HUVECs compared to the conditioned media from the vehicle-treated PE placentas (B, n = 6). Rosi and vehicle were cultured in placental media without any tissues for 24 h then applied to the HUVECs. Additionally, HUVECs cultured with standard full-serum media (HUVEC Only) all served as controls for this experiment. There were no significant changes in the number of nodes present among the HUVEC Only, Rosi, and vehicle conditioned media controls (C, n = 6). (Statistical analysis was performed by student’s t-test to determine significant differences between groups, ** p < 0.01, *** p < 0.001, bar plots and data reported are reported as numerical values ± SEM).