Figure 11.

There is a reduction of junctions present in HUVECs cultured with preeclamptic placental conditioned media and this was significantly increased after culture with Rosiglitazone-treated preeclamptic placentas. HUVECs were cultured with conditioned media on matrigel, and the number of junctions present was calculated by the Image J Angiogenesis Analyzer tool (30). Conditioned media from preeclamptic (PE) placentas show significantly reduced number of junctions as compared to healthy control placentas (A, n = 6). Conditioned media from Rosiglitazone (Rosi)-treated PE placentas led to a significant increase in the number of junctions present in the HUVECs compared to the conditioned media from the vehicle-treated PE placentas (B, n = 6). Rosi and vehicle were cultured in placental media without any tissues for 24 h then applied to the HUVECs. Additionally, HUVECs cultured with standard full-serum media (HUVEC Only) all as controls for this experiment. There were no significant changes in the number of junctions present among the HUVEC Only, Rosi, and vehicle conditioned media controls (C, n = 6). (Statistical analysis was performed by student’s t-test to determine significant differences between groups, * p < 0.05, *** p < 0.001, bar plots and data reported are reported as numerical values ± SEM).