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Cell proliferation of MCF-10A+Cu cells (normal MCF-10A cells cultured in a medium containing 25 µM CuCl2) was significantly reduced after treatment with curcumin following CTR1 knock-down. MCF-10A+Cu cells were first treated with curcumin or with specific siRNA against CTR1 (siCtr1) for 48 h and then with indicated concentrations of curcumin for 24 h. Values reported are ±S.E.M of three independent experiments. * p value < 0.05 when compared to respective control.
