Table 1.

Primers used for mutagenesis in this study.

Name	Sequence
R111A Forward	5′-GACAATGCTGCATACTTCTTTGCCTTGAAGTCCAAATGGATG-3′
R111A Reverse	5′-CATCCATTTGGACTTCAAGGCAAGAA GTA TGC AGC ATT GTC-3′
Y413F Forward	5′-GAAGATGAGCAGGAGCTCCACTTCGCTGTCCTACACTTCCACAAG-3′
Y413F Reverse	5′-CTTGTGGAAGTGTAGGACAGCGAAGTG GAGCTCCTGCTCATCTTC-3′
Y433F Forward	5′-CCAAAG GTCACCGACACTGAGTTCTCAGAAATCAAGATACACAAG-3′
Y433F Reverse	5′-CTTGTGTATCTTGATTTCTGAGAACTCAGTGTCGGTGACCTT TGG-3′
ICtrunc Forward	5′-CAGTTCCAGACAGGCATAGTTTGAGACCACCCTGCTGAGGCTGGC-3′
ICtrunc Reverse	5′-GCCAGCCTCAGCAGGGTGGTCTCAAACTATGCCTGTCTGGAACTG-3′

Note-Nucleotide changes introduced for generating each mutant are highlighted in bold.