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. 2022 Nov 2;119(45):e2206333119. doi: 10.1073/pnas.2206333119

Fig. 2.

Fig. 2.

Determination of functional antibody responses in the prime-only and prime-boost sera. Mice were vaccinated once or twice (4 wk apart) ID with 5 μg of monovalent mRNA-LNP or with 5 μg/antigen or 1.25 μg/antigen of the quadrivalent mRNA-LNP formulation. Negative control animals received 5 μg of Luc mRNA-LNP. Functional characterization of antibodies in the sera of vaccinated mice was assessed using MNT (A), NI (B) and ADCC reporter (C) assay against the A/Singapore/INFIMH-16-0019/2016 (H3N2, IVR-186) virus. MNT data are presented as endpoint titers of five randomly selected animals. ADCC data are presented as AUCs with a cutoff value of the average background plus five SDs. For MNT and ADCC data, bars represent the geometric mean for each group and error bars depict SD. Statistical significance was calculated using one-way ANOVA and groups were compared to the Luc control for their respective vaccination regimen (single/double vaccination). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NI data are presented as inhibition calculated against a virus-only control. Each data point represents inhibition of sera from five randomly selected animals and error bars are representative of SD. Complete lines represent data obtained from prime-only sera, whereas dashed lines represent data obtained from prime-boost sera.