Fig. 6.
Effects of QCT-stimulated autophagy on induction of apoptosis of SMMC7721 tumors in vivo. (A) The pictures of H&E staining, TUNEL and IHC staining for cleaved caspase-3 on tumor tissue sections. (B) The percentage of the necrotic area in the H&E staining images. (C) The percentage of apoptosis rate by quantifying TUNEL assay. (D) The quantitative analysis of IHC staining of cleaved caspase-3. (E–G) The representative images of Western blot analysis of bax, bcl-2 and cleaved caspase-3 proteins (E) and the densitometry analysis results of bax and cleaved caspase-3 (F) and bcl-2 (G). All the results are expressed as means±S.D. Within each panel, the value with a symbol “*” is significantly different from that of the control: *P<.05; **P<.01; ***P<.001. And the value with a symbol “#” is significantly different from that of the QCT group: #P<.05; ##P<.01; ###P<.001.