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. 2022 Oct 20;44(11):1263–1275. doi: 10.1007/s10529-022-03307-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Expression of PD-L1 is down-regulated in PD-L1 siRNA-transfected bone marrow-derived mesenchymal stem cells (BMSCs). A The expression of fluorescence-labeled PD-L1 in transfected bone marrow stromal cells was observed under optical and fluorescence microscope. Original magnifcation, × 400. B Western blotting analysis of PD-L1 protein in lentivirus-transfected BMSCs and levels of PD-L1 protein and mRNA in lentivirus-transfected BMSCs. C GFP-lentivirus transfected BMSCs under fluorescence microscopes. Original magnifcation, × 40. Data are expressed as means ± SD (n = 3). ***P < 0.001 vs. null-BMSCs