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. 2022 Oct 20;44(11):1263–1275. doi: 10.1007/s10529-022-03307-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Levels of IL-10 in colon tissues of each group were measured by enzyme-linked immunosorbent assay (Elisa) and quantitative real-time polymerase chain reaction (qRT-PCR) analyses of IL-10 mRNA expression in colons. B Western blotting analysis of phosphor-Akt (Ser473),phosphor-Akt (Thr308),AKT, phosphor-mTOR,mTOR and PTEN protein. C Levels of Ser473 protein and Thr308 protein in colon tissues of each group. D Levels of phosphor-mTOR protein in colon tissues of each group. E Levels of PTEN protein in colon tissues of each group. Data are expressed as means ± SD (n = 3). *P < 0.05,**P < 0.01, ***P < 0.001 vs. model group