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. 2022 Oct 31;12:1037523. doi: 10.3389/fonc.2022.1037523

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Copyright © 2022 Zheng, Song, Wang, Zhang, Tang, Wang, Li, Wu, Song and Xie

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of plasma exosomal tRFs. (A–E) The cleavage site of tRF-Leu-TAA-005, tRF-Asn-GTT-010, tRF-Ala-AGC-036, tRF-Lys-CTT-049, and tRF-Trp-CCA-057 on their own tRNA secondary structure. The levels of (F) tRF-Leu-TAA-005, (G) tRF-Asn-GTT-010, (H) tRF-Ala-AGC-036, (I) tRF-Lys-CTT-049, and (J) tRF-Trp-CCA-057 when incubated at room temperature. (K) qRT -PCR analysis of the five tRFs in the exosomes or isolated nucleic acids treated with RNase A. (L) Expression levels of the five tRFs from plasma exosome (EXO) and exosome-depleted supernatant (EDS) (**p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant).