Fig. 1.

Generation and validation of a conditional T-lymphocyte TH knockout model. A) Through crossing of TH loxP and Lck-cre mice to the F3 generation, 50% conditional genetic knockout (TH^T-KO) and 50% cre-negative (TH^Con) mice were generated, allowing for in vivo investigation of the role of TH within T-lymphocytes was generated. B) Representative DNA gel of respective samples following PCR amplification with oligonucleotide primers which produced differentially sized PCR products. C) Quantification of flow cytometry of TH mean fluorescence intensity (MFI) in splenic T-lymphocytes (CD3ε+) in TH^T-KO and TH^Con (N = 5 for each genotype); significance by Mann-Whitney U test, all values normalized to TH FMO tube.