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. Author manuscript; available in PMC: 2023 Oct 18.
Published in final edited form as: Brain Behav Immun. 2022 May 14;104:18–28. doi: 10.1016/j.bbi.2022.05.007

Fig. 4.

Fig. 4.

THT-KO animals have attenuated TH17 polarization following RSDS. Following RSDS or control paradigms, splenocytes were harvested for multiparametric flow cytometry for TH17 T-lymphocytes (CD3ε+CD4+CD8IL-17A+). Hierarchical gating strategy was employed to assess viability, percentage, and number of TH17 positivity. N = 5, 4, 5, 4 for respective genotypes and treatments. Two-way ANOVA Šidák multiple comparison tests of interest are listed if respective group or interaction effects were found to be significant.