TABLE 5.
The experimental data used for calibration of the model and validation of the simulated results.
| Type | Experiment | Cell/tissue type | Observation | Figure/Table | Preparation data |
|---|---|---|---|---|---|
| Calibration | Toepfer et al. (2020) | hiPSC-CMs | 33% increase of tension relaxation in R403Q | Figure 3C | hiPSC-CM cell lines at day 30 post-differentiation |
| Corrected tension relaxation due to MAVA in R403Q | |||||
| The fractional cell shortening in R403Q | Figure 3F | ||||
| (Green et al. (2016); Toepfer et al. (2020)) | hiPSC-CMs, Murine | Reduction in fractional cell shortening due to MAVA | Figure 3F | hiPSC-CM cell lines at day 30 post-differentiation, isolated adult rat ventricular cardiomyocytes treated with increasing concentrations of MAVA | |
| (Green et al. (2016); Awinda et al. (2020)) | Murine, Human | Reduction in maximum tension due to MAVA | Figure 3C | Isolated adult rat ventricular cardiomyocytes, skinned human myocardial strips | |
| Kampourakis et al. (2018) | Rat | Change in pCa50, Hill coefficient, and maximum tension due to OM and BLEB | Table 7 and Figure 4A | Demembranated rat ventricular trabeculae | |
| OM and BLEB dose-dependent contractile response | Figures 4D,E | ||||
| Validation | Sewanan et al. (2019) | hiPSC-CMs | The unaffected CaT in R403Q | Figure 3B | Engineered heart tissues made of hiPSC-CMs on decellularized porcine left ventricular tissue blocks |
| (Nag et al. (2015); Sarkar et al. (2020)) | Human, hiPSC-CMs | Negligible change in ATPase in R403Q | Figure 3D | Human β-cardiac myosin | |
| Green et al. (2016) | Murine | Unchanged CaT and pCa50 in Tension-pCa curve due to MAVA | Figure 3B, Fig. S1 | Isolated adult rat ventricular cardiomyocytes | |
| Rohde et al. (2018) | Human, Porcine, Bovine, and Murine | Reduction in ATPase rate due to MAVA | Figure 3D | Human β-cardiac myosin, Porcine and Bovine ventricular myosin, mouse cardiac myofibrils | |
| Kawas et al. (2017) | |||||
| Green et al. (2016) | |||||
| Gollapudi et al. (2021) | |||||
| Kawas et al. (2017) | Human & Bovine | Slowed relaxation in ATPase rate due to MAVA | Figure 3D | Human and bovine cardiac myosins | |
| Szentandrassy et al. (2016) | Canine | Change in APD due to 1 µM OM | Figure 5A | Left ventricular single canine myocytes | |
| (Bakkehaug et al. (2015); Utter et al. (2015)) | Murine Porcine | ATPase basal value increase due to OM | Figure 5B | Ex vivo mouse and in vivo pig hearts |