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GPC1 knockdown abolished LiCl driven β-catenin stimulation. OE19 cells were transfected with either LV-GPC1 or LVshGPC1#α in the presence of LiCl (40 mM) for 48 h. Western blot analysis of active p-GSK3-β, active p-β-catenin, TCF1/7 and c-myc. DMSO, dimethylsulphoxide; LiCl, lithium chloride; LV-GPC1, overexpressing GPC1 lentivirus; LVshGPC1#α, GPC1 knockdown lentivirus; GPC1, glypican 1.
