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. 2022 Oct;13(5):2082–2104. doi: 10.21037/jgo-22-240

Figure 4.

Figure 4

GPC1 regulates cell morphology. (A) Cell morphology of parental FLO1, GPC1 knockdown FLO1 cells was examined with immunofluorescence staining. Compared to spindle shaped fusiform structure of parental FLO1 cells the knockdown cells became more rounded, polygonal with enlarged binuclear and multinuclear morphology (white circle). (B) Reduced F-actin staining and its depolymerization into aggregates (white arrows) in GPC1 knockdown cells compared to negative control. (C) LV-GPC1 cells showed thick coarse actin bundles of stress fibers (white arrow) compared to empty vector control. Red indicates F-actin, green indicates e-GFP, blue indicates DAPI-stained DNA. Scale bar: 100 µm. LVshGPC1#α, LVshGPC1#β, and LVshGPC-1#γ, GPC1 knockdown plasmids; e-GFP, enhanced green fluorescent protein; DAPI, 4',6-diamidino-2-phenylindole-stained DNA; NC, negative scrambled control; EV, empty vector control; LV-GPC1, overexpressing GPC1 lentivirus; GPC1, glypican 1.