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. 1999 Jun;67(6):2920–2927. doi: 10.1128/iai.67.6.2920-2927.1999

FIG. 4.

FIG. 4

Detection of a reconstituted LPS-LKT complex by coimmunoprecipitation with anti-LKT MAb C6. Isolated LPS, SDS-PAGE LKT, and reconstituted LPS-LKT complex were incubated with anti-LKT C6 murine IgG MAb followed by precipitation with goat anti-mouse antiserum, and the precipitate was washed and subjected to analytical SDS-PAGE and silver staining. Lanes: 1, anti-LKT C6 murine IgG MAb and goat anti-mouse antiserum only; 2, CCS LKT (74 μg of LPS and 13 μg of protein); 3, isolated LPS (185 μg); 4, SDS-PAGE LKT (5.0 μg of protein); 5, reconstituted LPS-LKT complex (185 μg of LPS and 5.0 μg of protein); 6, molecular mass markers; 7, isolated LPS (18.5 μg); 8, SDS-PAGE LKT (1.0 μg of protein), respectively, directly subjected to analytical SDS-PAGE without immunoprecipitation to serve as markers for LPS and LKT, respectively.