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. 2022 Nov 14;106(24):8035–8051. doi: 10.1007/s00253-022-12279-1

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Fig. 3 — Expression and purification evaluation of the MBP:PLD:CP40 fusion protein. A Western blot using anti-polyhistidine antibody for the detection of MBP:PLD:CP40 and PLD:CP40. S—Molecular weight in kDa; 1—MBP:PLD:CP40 and PLD:CP40. B 15% SDS-PAGE evidencing the results of the fusion protein purification. S—Molecular weight in kDa. Washes were performed with different concentrations of imidazole: 1 – 40 mM; 2 – 60 mM; 3 – 80 mM, and 4—elution with 400 mM. Black arrows indicate the presence of the respective proteins