Table 1.

Physicochemical parameters, antigenicity, and allergenic potential of the MBP:PLD:CP40 fusion protein. The physicochemical parameters were analyzed using the ProtParam server. Antigenicity was analyzed on the ANTIGENpro server. The allergenic potential was analyzed using the AlgPred server

Parameter	Result
Allergenic potential	None
Antigenicity	ANTIGENpro = 0.846011
Number of amino acids	1039
Molecular weight	115.11 kDa
Theoretical isoelectric point	6.02
Instability index	22.80
Aliphatic index	74.28
GRAVY	− 0.525
Estimated half-life	30 h (mammalian reticulocytes, in vitro)
	> 20 h (yeast, in vivo)
	> 10 h (E. coli, in vivo)