Skip to main content
. 2022 Oct 31;13:1007022. doi: 10.3389/fimmu.2022.1007022

Figure 4.

Figure 4

Volcano plot of genes in the KEGG Ribosome and KEGG Cell Cycle sets in new-onset JDM cells compared to healthy pediatric controls and qRT-PCR validation of select Ribosome genes in treatment-naïve JDM patients vs age-matched controls (AMC). (A) Genes in the KEGG Ribosome (hsa03010) and Cell Cycle pathway (hsa04110) in JDM NK, T, and B cells compared to AMC. The Ribosome pathway has many ribosomal proteins while the Cell Cycle pathway contains genes involved in regulation of cell proliferation (listed in Table S6 ). Red dots indicate an adjusted P value of < 0.05 and blue dots indicate an adjusted P value of > 0.05. (B) qRT-PCR conformation of select genes in the KEGG Ribosome (hsa03010) pathway in JDM NK, T, and B cells compared to pediatric controls. deltaCT (dCT) was determined by CT of B2M – target CT.