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. 2022 Oct 31;13:1007022. doi: 10.3389/fimmu.2022.1007022

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Copyright © 2022 Hilliard, Throm, Pingel, Saucier, Zaher and French

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of NK cells of treatment-naïve JDM patients and age-matched controls. (A) Percentages of NK cells in total PBMCs and low ribosome expressing NK cells in the NK population in JDM PT and AMC. (B) qRT-PCR measurement of MALAT1 in sorted populations of NK, T, and B cells in treatment-naïve JDM PT compared to AMC. dCT was determined by B2M CT – MALAT1 CT. Representative histogram (C) and MFI (D) of pRPS6 as measured by flow cytometry in stimulated, enriched NK cells from treatment-naïve JDM PT and AMC. (E) NK Cell degranulation of treatment-naïve JDM PT and AMC in enriched NK cells unstimulated or stimulated with K562 cells. A representative flow plot is shown.