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. 1999 Jun;67(6):2964–2968. doi: 10.1128/iai.67.6.2964-2968.1999

FIG. 5.

FIG. 5

FIG. 5

Serum-pretreated B. subtilis organisms are phagocytosed by vitamin D3-induced U937 cells in a CD14- and LBP-dependent fashion. (A) Phagocytosis was performed at 37 or 4°C in the presence or absence of 2.5 μg of murine recombinant LBP per ml. Internalization of the labeled bacteria is inhibitable with Fab fragments of the anti-CD14 MAb biG 14 or with a 20-fold excess of unlabeled bacteria (added where indicated). The ratio of cells to labeled bacteria was 1:50. (B) The same experiment as described for panel A was carried out with uninduced U937 cells, which do not express CD14.