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As a marker of neutrophilic infiltration, colonic MPO activity was increased in all colonized rats with respect to GF controls. Homogenized cecal tissues collected 1 month after bacterial colonization were assayed by a colorimetric biochemical assay for MPO (see Materials and Methods). ∗, P < 0.03 with respect to E. coli and P < 0.0001 with respect to GF rats. §, P < 0.0001 with respect to GF rats and P < 0.01 with respect to DESEP-B.
