FIG. 5.

RgpA or Kgp do not cleave the HLA-DR molecule on HUVE cells. HUVE cells were seeded at a density of 10⁵ cells/cm² and incubated for 3 days in supplemented medium containing 3 nM rIFN-γ to induce HLA-DR expression. Cells were then washed and cultured for an additional 24 h in serum-containing supplemented medium with 60 nM RgpA or Kgp. The cells were subsequently removed, washed, and solubilized in SDS; the proteins were then resolved by SDS-PAGE and subjected to Western blot analysis. (A) Detection of the HLA-DR α chain. (B) Detection of the HLA-DR β chain. Lane 1, untreated HUVE cells; lane 2, RgpA treatment of HUVE cells; lane 3, Kgp treatment of HUVE cells. The data are representative of three separate experiments.