Fig. 4.

BDNF Skeletal Muscle Protein and Transcript Response to 80% Acute Aerobic Exercise. A) Representative western blot probing for BDNF before, 30-minutes post and 3-hours post 80% acute AE. All samples were normalized to total protein stain. Protein and transcript content of B) Mature BDNF protein, C) ProBDNF protein, D) Total BDNF protein and E) Pro:Mature BDNF protein ratio and F) BDNF transcript n = 15 (8M/7F). Mature BDNF is expected at 15 kD, Pro BDNF is expected at 30 kD. All data are represented as mean±SEM. n = 13 PRE (7M/6F), n = 8 POST (5M/3F), and n = 8 3 h (4M/4F). ^*p < 0.05 vs PRE, ^**p < 0.01 vs PRE, ^#p < 0.05 main effect of time.