Figure 5.

Increased structural constraints are associated with gene duplication status. (A) Two-dimensional density plot of sequence constraint (as mean conservation score) versus structural constraint (as mean mutation score by (45)⁠ for the same regions of 200 bp to 50bp upstream of the TSS for all yeast genes (overall Spearman's rho = −0.229, P ≤ 10⁻⁵⁷). Each line corresponds to a linear regression fit. Shaded band corresponds to standard error. (B) Left: two-dimensional scatterplots of gene pair co-expression, measured as scaled Adjusted co-expression Score (obtained from SPELL) against nucleosome positioning correlation, measured as the Pearson correlation coefficient of nucleosome occupancy profiles between gene pairs. Right: Gene pair co-expression against TF similarity measured as the Jaccard index of TFBS found in the promoters of each gene pair. Red: SSD gene pairs, Green: WGD gene pairs. Size of points proportional to the estimated diversity between the genes in each pair.