Table 1. Sequences and Binding Affinities of Fluorescein-Labeled Analogues Bicycle Families Identified through Phage Display.
family | Bicycle number | screening stage | sequence | affinity Kd (nM) |
---|---|---|---|---|
1 | 1 | Naïve | ACPFGCHTDWSWPIWCA(Sar6)K(Fl) | 508 |
2 | Affinity maturation | ACPFGCMKNWSWPIWCTNK(Sar6)K(Fl) | 20.0 | |
2 | 3 | Naïve | ACWPLDSYWCARICA(Sar6)K(Fl) | 208 |
4 | Affinity maturation | QKWCAPLSDYWCNRICA(Sar6)K(Fl) | 65.5 | |
3 | 5 | Naïve | ACVTTSYDCFLHLLGCA(Sar6)K(Fl) | 888 |
6 | Affinity maturation | ACVTTSYDCWVTLGHCSRF(Sar6)K(Fl) | 242 |