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. 2022 Oct 7;65(21):14337–14347. doi: 10.1021/acs.jmedchem.2c00065

Table 1. Sequences and Binding Affinities of Fluorescein-Labeled Analogues Bicycle Families Identified through Phage Display.

family Bicycle number screening stage sequence affinity Kd (nM)
1 1 Naïve ACPFGCHTDWSWPIWCA(Sar6)K(Fl) 508
  2 Affinity maturation ACPFGCMKNWSWPIWCTNK(Sar6)K(Fl) 20.0
2 3 Naïve ACWPLDSYWCARICA(Sar6)K(Fl) 208
  4 Affinity maturation QKWCAPLSDYWCNRICA(Sar6)K(Fl) 65.5
3 5 Naïve ACVTTSYDCFLHLLGCA(Sar6)K(Fl) 888
  6 Affinity maturation ACVTTSYDCWVTLGHCSRF(Sar6)K(Fl) 242