Figure 2.

Increased protein expression of select UPR^mt markers in the absence of ATF5 with no changes in autophagy or lysosomal proteins in whole muscle. A) Representative blots of UPR^mt proteins in whole muscle with corresponding Ponceau stains. Proteins include chaperones mtHSP70, HSP60, Cpn10, the protease LONP1, and transcription factors ATF4 and CHOP. B) Representative blots of basal autophagy proteins in whole muscle, including cytosolic markers Atg7, Beclin-1 and lysosomal markers LAMP1 and V-ATPase with Ponceau stains. Corresponding quantifications of C) UPR^mt and D) autophagy and lysosomal proteins corrected for Ponceau (n = 8–10). ∗P < 0.05, unpaired t-test. A. U., arbitrary units; KO, knockout; S, standard; WT, wild-type.