Figure 3.

The muscle of ATF5 KO mice presents a more abundant mitochondrial pool with reduced organellar function. A) Representative blots in whole muscle of the transcription factor involved in mitochondrial biogenesis PGC-1⍺, the mitochondrial marker VDAC and corresponding Ponceau stains. B) Quantifications of PGC-1⍺ and VDAC corrected for Ponceau (n = 8–10). Mitochondrial content was assessed from C) COX Enzyme Activity values (n = 9–10) and D) Mitochondrial Yields (SS and IMF combined) derived from mitochondrial isolation experiments (WT: n = 18, KO: n = 27). Mitochondrial respiration in E) SS and F) IMF mitochondria expressed in natoms O₂/mg/min in both passive (State IV) and active (State III) respiratory conditions. Organelle function was also assessed by measuring ROS emission in G) SS and H) IMF subfractions (n = 18–27). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 unpaired t-test, WT vs KO basally or in given respiratory state. A. U., arbitrary units; KO, knockout; S, standard; WT, wild-type.