Figure 4.

ATF5 regulates the expression of glycolytic and antioxidant enzymes, in addition to apoptotic cytochrome c release. A) Representative blots in whole muscle of the glycolytic enzymes GAPDH, PFK-1, LDHA and the β-oxidation protein CPT1b with Ponceau stains. B) Quantifications of the enzymes corrected for loading control or Ponceau (n = 7–10). C) Representative blots of NQO1 and HO-1 in whole muscle samples with respective Ponceau stains, and MnSOD with VDAC in SS mitochondria. D) Quantifications of antioxidant proteins corrected for Ponceau stains or the respective loading control (n = 6–9). E) Representative blot of cytochrome c and PFK-1 in cytosolic samples. F) Quantification of cytosolic cytochrome c protein corrected for PFK-1 (n = 8). An amount of 5 $\mu$g was loaded to probe for GAPDH and was normalized to Aciculin, while 25 $\mu$g was used to target the other proteins and were normalized to Ponceau stains. ∗P < 0.05 unpaired t-test. A.U., arbitrary units; M, IMF mitochondrial sample; KO, knockout; S, standard; WT, wild-type.